Protokoly

ELISA

ELISA-Peptide Assay Protocol (ELISA-P) Cell Signaling Technology (CST) Enzyme Immuno Assay Protocol (EIA) Cell Signaling Technology (CST) HRP Chemiluminescence ELISA-P (HRP Chemiluminescent ELISA-P) Cell Signaling Technology (CST) PathScan® Sandwich ELISA Protocol (Chemiluminescent ELISA) Cell Signaling Technology (CST) PathScan® Sandwich ELISA Protocol (Chemiluminescent ELISA - for kits with Lyophilized Antibodies) Cell Signaling Technology (CST) PathScan® Sandwich ELISA Antibody Pair Protocol (ELISA-Pair) Cell Signaling Technology (CST) PathScan® Sandwich ELISA Protocol (Colormetric ELISA) Cell Signaling Technology (CST) PathScan® Sandwich ELISA Protocol (Colormetric ELISA - for kits with Lyophilized Antibodies) Cell Signaling Technology (CST)

Western Blot

General western blot protocol Abcam Sodium azide removal protocol Abcam Biotin conjugation Abcam Western Immunoblotting Protocol  Cell Signaling Technology (CST) Western Immunoblotting (Fluorescent) Protocol  Cell Signaling Technology (CST) Western Blot Reprobing Protocol (Western / Reprobe) Cell Signaling Technology (CST) Western Immunoblotting Troubleshooting Guide Cell Signaling Technology (CST) Changes to Recommended Western Blotting Protocols Cell Signaling Technology (CST) In-Cell Western Protocol (In-Cell Western) Cell Signaling Technology (CST)

Imunofluorescence

Immunocytochemistry and immunofluorescence protocol Abcam ISH: in situ hybridization protocol Abcam Immunofluorescence Protocol (IF Standard) Cell Signaling Technology (CST) Immunofluorescence Protocol with Methanol Fixation Cell Signaling Technology (CST) Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm) Cell Signaling Technology (CST)

Imunohistochemie

IHC staining protocol for paraffin, frozen, and free floating sections Abcam Immunohistochemistry Protocol (Frozen) for SignalStain® Boost Detection Reagent (IHC / Frozen) Cell Signaling Technology (CST) Immunohistochemistry Protocol (Paraffin) for SignalStain® Boost Detection Reagent (IHC / Paraffin) Cell Signaling Technology (CST) Fluorescent Multiplex Immunohistochemistry Protocol (mIHC/Paraffin) Cell Signaling Technology (CST) Immunohistochemistry Troubleshooting Guide Cell Signaling Technology (CST)

Průtoková cytometrie

Counting cells using a hemocytometer Abcam Direct flow cytometry protocol Abcam Flow Cytometry Protocol (Flow) Cell Signaling Technology (CST) Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood (Flow Alternate) Cell Signaling Technology (CST) Flow Cytometry Troubleshooting Guide Cell Signaling Technology (CST)

ChIP

Chromatin immunoprecipitation (ChIP) tips and tricks Abcam ChIP troubleshooting tips Abcam ChIP using plant samples: Arabidopsis Abcam Chromatin preparation from tissues for chromatin immunoprecipitation (ChIP) Abcam Cross-linking chromatin immunoprecipitation (X-ChIP) protocol Abcam Native chromatin immunoprecipitation protocol Abcam Cross-linking chromatin immunoprecipitation (X-ChIP) protocol Abcam SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads) (ChIP Agarose) Cell Signaling Technology (CST) SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic) Cell Signaling Technology (CST) SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads) (ChIP Agarose) Cell Signaling Technology (CST) SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic) Cell Signaling Technology (CST) Chromatin Immunoprecipitation Troubleshooting Guide Cell Signaling Technology (CST)

Imunoprecipitace

Immunoprecipitation (IP) protocol Abcam Immunoprecipitation Protocol / (For Denatured Proteins)  Cell Signaling Technology (CST) Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein) Cell Signaling Technology (CST) Immunoprecipitation Protocol Utilizing Magnetic Separation / (For Analysis By Western Immunoblotting) (IP Magnetic) Cell Signaling Technology (CST) Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Immobilized IP) Cell Signaling Technology (CST)