KMT1E/SETDB1 Polyclonal Antibody

ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4; Histone-lysine N-methyltransferase SETDB1; ERG-associated protein with SET domain; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SETDB1; KIAA0067

Histone methyltransferase that specifically trimethylates 'Lys9' of histone H3. H3 'Lys9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zincfinger proteins. Probably forms a corepressor complex required for activated KRASmediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumorrelated genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1TRIM28ZNF274 complex may play a role in recruiting ATRX to the 3'exons of zincfinger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).